Hi, currently in our lab theres a lot of transgenic mice model generation, wich means a lot of breeding and lot of genotyping. We are using tails, we lyse them ON and run a PCR and a gel. The problem is since we got up to anywhere from 20 to 100 tails a day and they have to be genotyped for up to 6 genes this method is proving to be very time consuming.
Im trying to find a streamlined method that dosent involve so much man hours to complete.
P.S : we just have una qPCR machine but 3 normal thermocyclers dedicated to genotyping.
Can you multiplex primer sets to minimise sample preparation and the number of pcrs?
I guess much of the work is in loading all the gels? You could develop qPCR probes for your six genes. Then you could scratch most of the gels, save for the occasional quality control.
- Badges
- Science method