Does anyone know papers that use large databases of abundance of different groups of species?

03 October 2020 2,506 4 View

What would be the better buffer to bind Mouse IgG1 to a Protein A affinity colum?

10 November 2016 1,847 6 View

Does anyone have a protocol to purify untagged Streptavidin?

10 November 2016 1,352 3 View

It is more important to optimize DNA sequence in yeast or mammalian expression of proteins?

10 November 2015 2,053 1 View

Are there any tips to improve the binding of GST-tagged proteins to a GST affinity column?

09 October 2015 3,458 4 View

Does anyone know a protocol to dephosphorylate proteins from a crude cell extract?

We are expressing a protein by transient tranfection in Hek 293 cells. When performing western blot analysis against our protein of interest (PAH), we obtain two bands, being, probably, our...

02 March 2015 4,433 8 View

Why do I get a double band when performing western blot analysis of the PAH protein in Hek 293 cells?

01 February 2015 7,331 3 View

How would you explain a decline in the fluorescence observed in a Real-Time reverse transcription PCR when starting from a very diluted RNA sample?

01 February 2015 7,807 6 View

Interested in a job position at the Institute of Ecology in Xalapa, Mexico?

Http://www.inecol.mx/inecol/index.php/es/ct-menu-item-1/ct-menu-item-19/one-research-position-in-the-research-network-of-multitrophic-interactions

07 August 2013 4,617 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View