How to measure neurotransmitters with HPLC?

Hi everyone, Please can somebody help me? I would like a protocol and a method how to measure the neurotransmitters using HPLC (adrenaline, noradrenaline, serotonin, dopamine, glutamate) Thank...

12 March 2016 3,555 5 View

Can anyone use two sampling designs in a case control study?

In a case control study, systematic sampling design for case (with disease) and purposive sampling design for control (without disease non -case healthy individual) can both design be used?

14 December 2015 8,330 6 View

How can I reduce ketones by Daucus carota?

19 May 2015 9,421 4 View

What is the three dimensional external graphical representation of black hole?

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26 February 2015 8,461 3 View

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21 December 2014 4,409 4 View

Are there any researchers able to collaborate on the topic of transmutation of long lived fission product in fast reactor?

Hi My research area is the transmutation of long lived fission product in fast reactorI am looking for a collaboration in the same research axis

14 November 2014 2,496 1 View

How to extract the bioavailable chromium from the soil?

I would like to measure the bioavailable chromium of some soil. How do I go about extracting it? I have alkaline soil (with a pH of about 8.5).

11 March 2014 7,490 7 View

How to identificate the structure of the textile colorants?

I have the references of this colorants, but I'd like to find their structures

01 January 1970 3,736 7 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Is there such a thing as Igg specific MHC class II epitopes or do all non-self MHC class II epitopes trigger antibody production of all subtypes?

I know there are bioinformatic tools that allow epitope prediction to determine if a certain peptide would trigger an immune response. I also read that for allergen diagnoses IgE of sensitised...

09 February 2021 4,173 3 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View