Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

How many GCMs exist currently? Which are the most prominent ones among them?

General Circulation Models or Global Climate Models are over 40 when I went through a research article long back. I would like to know how many GCMs are currently in use. Names of them aren't what...

03 August 2020 2,789 5 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

Is groundwater content considered in SM2RAIN algorithm?

I have come across SM2RAIN algorithm and can't help but wonder about groundwater content. I couldn't notice its inclusion in the algorithm. Is it something I've missed or I need to refer further?...

09 June 2020 5,272 6 View

How to do regridding using MATLAB?

I have a batch of netCDF files of precipitation data at 30km * 30km resolution. I attached a sample file of it. I want to convert them to 10km * 10km resolution using MATLAB. I tried using...

12 May 2020 6,007 6 View

What are the uses of extreme values in Hydrological aspect?

For the research work I'm working on, I came across usage of extreme values while studying about deriving IDF curves for future climatic scenarios. What kind of results can I produce with extreme...

27 January 2020 4,145 4 View

What are the alternatives of Global Climate Models? Apart from downscaling, any methods exist to derive local climate variables from GCMs?

GCMS are used to predict atmosphere and climate behavior. Apart from GCMs, are there any methods to predict climate behavior? In GCMs particularly, are there any methods to derive local-scale...

02 January 2020 7,752 14 View

What the concentration should taken of Traut's Regents and Peptide?

i am coupling 31 amino acid sequences to PEG, before that i have to activated this peptide with traut's reagents. Can anyone suggest me the which molar equivalent of concentration of both chemical...

07 April 2019 6,334 1 View

How to perform Cytotoxicity activity of Plant Extract (essential oil) without taking of 100% DMSO?

My super critical fluid extracts (SFE) are immiscible in 1 % DMSO for carrying out Cytotoxicity assay SFE is essential oil so i have problem to mix the oil and 1 % DMSO due to different polarity...

20 March 2019 2,037 2 View

Can anyone suggest me method for the coupling of Peptide with DPSE-PEG-MAL?

1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine- N-[Maleimide (Polyethylene Glycol)2000] ammonium salt [DSPEPEG- MAL] is poymer and I want to conjugate with some peptide. So Please suggest me any...

13 November 2018 2,081 1 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

What is the current status of black female police officers?

I am a doctoral candidate at Walden University. My dissertation is centered on the lived experience of black female police officers and their perspective of excessive use of force administered...

29 December 2020 8,452 1 View

Will automated killing diminish the error rate by police?

10 December 2020 5,548 3 View

How to personalize math learning according to profiles?

Hello, I am interested in the personalization of learning based on profiles, more specifically in mathematics. Do you know any relevant references? Thank you

11 October 2020 4,473 0 View

After the shocking case of George Floyd, should the physical restraint techniques used in the police and psychiatric settings be recoded?

George Floyd's case shocked the world. Physical restraint is a method used by the police but also in the management of psychiatric patients who pose a danger to themselves or others. In your...

10 June 2020 1,331 9 View

Are human rights activists biased?

When they support one that matching to their mind set-up or ideology, they might overlook the right of other one.... E.g. violent protester vrs. police.

25 December 2019 4,280 13 View

Research Instrument recommendation - Police view on IPCMC?

I'm doing a research on police's perception/view on a government policy (IPCMC) which had not been implemented yet. Is there any suitable survey instrument or questionnaire I can, might use?

04 May 2019 1,480 1 View

Can anyone recommend articles looking at organisational stressors within the police (eg paperwork) and the effect this has on their job performance?

I am writing a dissertation on the causes of stress in the police, and the effect this has on police mental health. struggling to find any good references

03 April 2019 5,777 2 View

Does anyone have a lead on sites I could go to that might be connected, in some way to my Dissertation Topic, Guns in the Hands of Teachers?

My topic will look at surveys disseminated to students, teachers, parents, community groups, NRA, police, Department of Education.

03 April 2019 9,197 4 View