Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

How many GCMs exist currently? Which are the most prominent ones among them?

General Circulation Models or Global Climate Models are over 40 when I went through a research article long back. I would like to know how many GCMs are currently in use. Names of them aren't what...

03 August 2020 2,789 5 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

Is groundwater content considered in SM2RAIN algorithm?

I have come across SM2RAIN algorithm and can't help but wonder about groundwater content. I couldn't notice its inclusion in the algorithm. Is it something I've missed or I need to refer further?...

09 June 2020 5,272 6 View

How to do regridding using MATLAB?

I have a batch of netCDF files of precipitation data at 30km * 30km resolution. I attached a sample file of it. I want to convert them to 10km * 10km resolution using MATLAB. I tried using...

12 May 2020 6,007 6 View

What are the uses of extreme values in Hydrological aspect?

For the research work I'm working on, I came across usage of extreme values while studying about deriving IDF curves for future climatic scenarios. What kind of results can I produce with extreme...

27 January 2020 4,145 4 View

What are the alternatives of Global Climate Models? Apart from downscaling, any methods exist to derive local climate variables from GCMs?

GCMS are used to predict atmosphere and climate behavior. Apart from GCMs, are there any methods to predict climate behavior? In GCMs particularly, are there any methods to derive local-scale...

02 January 2020 7,752 14 View

What the concentration should taken of Traut's Regents and Peptide?

i am coupling 31 amino acid sequences to PEG, before that i have to activated this peptide with traut's reagents. Can anyone suggest me the which molar equivalent of concentration of both chemical...

07 April 2019 6,334 1 View

How to perform Cytotoxicity activity of Plant Extract (essential oil) without taking of 100% DMSO?

My super critical fluid extracts (SFE) are immiscible in 1 % DMSO for carrying out Cytotoxicity assay SFE is essential oil so i have problem to mix the oil and 1 % DMSO due to different polarity...

20 March 2019 2,037 2 View

Can anyone suggest me method for the coupling of Peptide with DPSE-PEG-MAL?

1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine- N-[Maleimide (Polyethylene Glycol)2000] ammonium salt [DSPEPEG- MAL] is poymer and I want to conjugate with some peptide. So Please suggest me any...

13 November 2018 2,081 1 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

How to calculate time needed to evacuate a pressure from a tank using a pump?

Dear I have an air reservoir with 50 bar pressure , this reservoir is fed by 2 bar air pressure source, I would like to instal air pump ( 30 m3/h ) to evacuate the 2 bar fed pressure so I always...

06 February 2021 3,520 3 View

Is diversity in teaching methods for students important in communicating information?

Is diversity in teaching methods for students important in communicating information?

26 January 2021 3,387 7 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

Corpus linguistics collocate indicative of diversity?

I have a question, as to whether collocations in corpus linguistics can be used to indicate diversity. I have a corpora of media news articles, and I manage to find out the frequency of my target...

19 January 2021 1,446 1 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

Permeability of fractured reservoirs?

Hi all, What I know the permeability of matrix and fracture can be in a broad range in fractured reservoirs. Am looking for the most usual range for their permeability separately. I will be highly...

15 January 2021 5,271 2 View

R package for multivariate dispersion as a measure of beta diversity?

Hello, everyone: I've been reading some old and new papers on how to compute beta diversity using incidence or abundance data from several assemblages/communities. For my data, I am interested...

03 January 2021 9,293 1 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View