907-R and 1429-R primers were used as sequencing primers instead of 27-F and 1429-R. Reasons given were that 907-R covers the same data region with 27F but in opposite direction. And, it binds internally on the fragment and gives better data quality. My troubleshooting showed that with no overlapping, I cannot make a consensus sequence nor generate a contig. Hence, I need help. Does anyone has a clue on any publication related to this or other suggestions? Thanks.