I am planning to edit the binary vector "pCAMBIA 1301" to insert a new gene and will be cutting out the right border in the process. My plan is to reintroduce the right border along with the ~100bp of sequence that would be cut out by my digestion in order to preserve any overdrive sequence (T-DNA transfer enhancing sequence).
Upon searching though, I wasn't really able to find the overdrive sequence in the pCAMBIA 1301 vector and was thinking it might improve T-DNA excision/transfer to instead introduce one I found in literature.
Can anyone comment on the importance of the overdrive sequence following right borders in Ti plasmids?
The only papers I have found are rather dated and I could use some current, real life commentary.
Hello Jackie, i think your reason for ignoring the papers at your disposal is somewhat lame. That a paper is dated does not in anyway remove from it the authority it has on the subject matter. Dated papers by Lowry (1956) and Bradford (1972) are still forces to reckon with in their subject areas. That said, I recommend you go through the published thesis of Seokhyun Lee (2010) and the following attachments. I hope they help you out.
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