Impact of metalaxyl fungicide on soil microbes?

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Program to find gene copy number in genome?

I am trying to find out how many copies of a gene that I have primers for are in a training set of genomes. I would like program where I enter the genome and the primer (that has degenerate...

15 November 2020 4,523 3 View

Do you have an endnote style for Marine Ornithology?

Any seabird folks out there have an endnote style file for Marine Ornithology? Nothing available online, trying to avoid creating one from scratch - thanks! Perhaps we can make the file publicly...

01 November 2020 9,642 7 View

Storing environmental data with PostgreSQL?

I am wondering if anyone uses postgresql to store data collected by continuous sensors and if there is a pipeline that makes this easier? Also, any other general tips with postgresql would be very...

18 August 2020 939 2 View

Is it necessary to do elution of proteins from Polyacrylamide gel?

Dear researchers, I would like to ask is it necessary to do Elution of proteins from Polyacrylamide gel before sending the protein sample to Nano-LC-TOF-MS Analysis? This is what I plan, I would...

01 August 2020 3,680 3 View

Aside from Mendeley, what other tools/apps can help researchers organize their citations and references?

My question is on looking for suggestions on how citations and references can be easier to generate. Thanks for the response. Hop your sharings could help me.

31 May 2020 5,026 14 View

Hi, i am conducting a legal research on; why people in Ghana are reluctant to commence tort actions like battery in court?

would be very grateful if some recommendations are made on the relevant cases or articles to read to help. thank you.

20 April 2020 4,801 3 View

Regarding on metabolomic?

Dear researchers, May I know how to know what metabolites will be produced by the cells from experiment (Wetlab): 1) Cell (cell culture) 2) Cell infected with virus 3) Cell treated with plant...

31 March 2020 5,865 3 View

How to make elution buffer for SDS-PAGE?

Dear researchers, How to make elution buffer for SDS-PAGE? There is a lot of protocol to make elution buffer, but they didn't show the amount of each ingredient needed. thank you.

25 March 2020 8,333 4 View

Elution of protein from SDS PAGE ?

Dear researchers, May I know do anyone can suggest the protocol of protein elution from SDS PAGE ? Any simple and inexpensive methods? Thank you.

24 March 2020 2,073 13 View

Regarding on Proteomics ?

Dear researchers, I'm currently doing a study regarding on antiviral mechanism of selected plant using metabolomic approach. Plant extract (fruit) will be used and the virus model will be used is...

21 March 2020 5,885 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,557 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

How can I prepare 300mg/L concentration of lead(II) nitrate ( Mol. Wt reffered is 331.21) incoorporated Luria-Bertani (LB) agar ?

I need to check the Heavy metal (lead) consumption assay using microbes at varying concentration in same media. ( 200mg/L to 2000mg/L).kindly help me to clear it.

19 January 2021 1,946 9 View

Why according to FRAC (fungicide resistance action committee) phenylamide fungicides application interval should not exceed 14 days?

Why according to FRAC (fungicide resistance action committee) phenylamide fungicides application interval should not exceed 14 days. Anyone know reason behind this strategy

18 January 2021 1,166 3 View