Hello everyone,

I need to do some immunolabelling on the optic nerve head in order to look for elastin and fibrillin-1 and I am struggling on finding good antibodies. I am not going to use rabbit's as they give lots of fluorescent background so I am looking for mouse primary antibodies, mainly.

I searched the literature and there are many immunolabelling on optic nerve head cells, but I am actually doing immuno on tissue.

I am going to embed the tissue first (optic nerve head) and then cut thin section (10 microns) with the cryostat. 

Does anybody have any idea on which antibody may work on this kind of experiment? Does anybody know any literature who has done a similar experiment, providing good immunolabelling images?

There are lots in the internet but none can completely satisfy. Besides, mono or polyclonal?

Thank you