Laura Paletto

6 Questions 10 Answers 0 Followers

Questions related from Laura Paletto

How axons look like when stained with osmium tetroxide?

Hi everyone, I am running experiments where I used osmium tetroxide to stain axons in the optic nerve and then image it with x ray microtomography. I don't know if my experiment worked as it seems...

07 March 2019 8,229 13 View

Amira and Image j: how can I reconstruct a 3D tissue?

Hi all, I've been starting using Amira for image analysis and I am having issues on aligning and subsequently merging 5 stacks. I need to have these 5 stacks completely aligned (these are from the...

05 May 2018 197 1 View

Change in the shape of the spectrum, how to quantify?

Hello, I am currently working on assessing the elastin spectrum properties in different tissues and I'd like to compare the elastin spectral signature between tissues to see whether or not there...

14 July 2017 7,660 6 View

Quantify Second harmonic and two photons signals?

Hello, I'll need to quantify, somehow, the SHG (second harmonic generation) signal within collagen fibres and TPEF (two-photons excited fluorescence) signal within elastic fibres. Does anyone...

12 April 2017 7,920 1 View

Elastin Fuorescence

Hello everyone, I am working on elastin autofluorescence and I am using rat aorta to optimise a protocol. I have read from literature that elastin fluoresces by itself which makes difficult to...

06 March 2017 5,878 3 View

Are there any sticky microscope spacers without leaking the liquid?

Hello, I am working with optic nerve sections and I am trying to image them using a new method which does not involve mounting the sample on a slide and cover it with a coverslip. I am placing the...

10 September 2016 2,206 7 View