11 Questions 13 Answers 0 Followers
Questions related from Laura Paletto
Hi all, does anyone know how specifically Lugol's iodine and Tannic acid enhance the connective tissue signal for EM work? Where do they bind into the proteins? I cannot find any specific ref for...
11 March 2019 9,265 0 View
Hi everyone, I am running experiments where I used osmium tetroxide to stain axons in the optic nerve and then image it with x ray microtomography. I don't know if my experiment worked as it seems...
07 March 2019 8,283 13 View
Hi all, I've been starting using Amira for image analysis and I am having issues on aligning and subsequently merging 5 stacks. I need to have these 5 stacks completely aligned (these are from the...
05 May 2018 239 1 View
Hi Everyone, I am using spectral imaging to determine the elastin spectral signature in my sample and I applied the spectral unmixing to separate different component within the same sample that...
08 February 2018 4,023 2 View
Hello, I am currently working on assessing the elastin spectrum properties in different tissues and I'd like to compare the elastin spectral signature between tissues to see whether or not there...
14 July 2017 7,720 6 View
Hello, I'll need to quantify, somehow, the SHG (second harmonic generation) signal within collagen fibres and TPEF (two-photons excited fluorescence) signal within elastic fibres. Does anyone...
12 April 2017 7,956 1 View
Hello everyone, I am looking into the spectral properties of elastin in young, mid age and old donor's samples. How I can quantify this in order to be able to determine differences (in any) in the...
21 March 2017 1,511 0 View
Hello everyone, I am working on elastin autofluorescence and I am using rat aorta to optimise a protocol. I have read from literature that elastin fluoresces by itself which makes difficult to...
06 March 2017 5,904 3 View
Hello everyone, I need to do some immunolabelling on the optic nerve head in order to look for elastin and fibrillin-1 and I am struggling on finding good antibodies. I am not going to use...
30 January 2017 2,366 1 View
Hello, I am working with optic nerve sections and I am trying to image them using a new method which does not involve mounting the sample on a slide and cover it with a coverslip. I am placing the...
10 September 2016 2,238 7 View
I am imaging some optic nerve head sections using non linear microscopy (SHG/TPEF simultanously). I am going to merge the two channels (SGH green and TPEF red) in order to see the co-localisation...
23 August 2016 4,643 2 View
