Could someone please help me with cloning, particularly for shRNAs?

Hi everyone, my labmate and I have been trying to clone a few shRNA constructs in the past 2 months. We encountered quite a few issues and only successfully cloned 2 out of 18 constructs so far. I...

08 September 2016 822 4 View

How do I explain round circles appearing in my hippocampal neuronal culture?

02 March 2015 3,920 12 View

What can trigger AMPAR endo/exocytosis?

Hi all, I am using the Super Ecliptic pHluorin-GluA1 (or 2) probe to study the dynamic of AMPAR. Yet the treatment I am using as a positive control (chemical LTP: glycine, bicuculine, strychnine)...

10 November 2014 9,109 5 View

Could anyone recommend a blue (excitation at 405 or 350) tagged anti-chicken antibody?

Hi all, Do you know of any good blue tagged anti-chicken antibody for immunofluorescence on cultured neurons or other cell types? I am doing an immunocytochemistry assay that needs all 4 colors:...

09 October 2014 9,572 5 View

Do you have a good protocol/antibody to stain for surface AMPA receptor?

09 October 2014 8,019 6 View

Can anyone help with recording AMPAR mediated mini EPSC in dissociated hippocampal cultured?

I just started doing electrophysiology since last year and now interest in learning about the zinc sensitivity of AMPAR mediated mini EPSC. For this goal, I am trying to record from dissociated...

03 April 2014 1,524 20 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View