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Hi everyone, my labmate and I have been trying to clone a few shRNA constructs in the past 2 months. We encountered quite a few issues and only successfully cloned 2 out of 18 constructs so far. I...
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Hi all, I just moved to a new lab and started cell cultures again. We are culturing hippocampal neurons from E18 rat embryos. The cells seem to mature at normal rate and I can see synaptic...
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Hi all, I am using the Super Ecliptic pHluorin-GluA1 (or 2) probe to study the dynamic of AMPAR. Yet the treatment I am using as a positive control (chemical LTP: glycine, bicuculine, strychnine)...
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Hi all, Do you know of any good blue tagged anti-chicken antibody for immunofluorescence on cultured neurons or other cell types? I am doing an immunocytochemistry assay that needs all 4 colors:...
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Hi all, I am interested in looking at the surface AMPA receptors in cultured hippocampal neurons after overexpressing another protein of interest. I am planning to look at both GluR1 and other...
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I just started doing electrophysiology since last year and now interest in learning about the zinc sensitivity of AMPAR mediated mini EPSC. For this goal, I am trying to record from dissociated...
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Thanks a lot for the invitation. I'm setting up an Immuno-PCR assay to detect HPV protein. I would like to talk about IPCR, PCR, real-time PCR, etc.
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