I am planning to perform an antibody-ligand interaction study with SPR/Biacore. The idea is to immobilize my antibody with Protein A/G. It would be great if you could share your comments, what to consider, advantages/disadvantages of Protein A/G immobilization for SPR.
I recommend you to buy the protein A sensor chip instead to made your own protein A sensor chip. Because some signal drift could be observed after the amine-coupling of protein A. The protein A is commonly used compared to protein G.
For my personal experience, I prefer to use the igG kit or Fab kit using a regular CM5 sensor chip. Using this kit you are not worry about the regeneration buffer and these kits show reproducible data.
The use of protein A sensor technology, It is commonly used Better yet to buy a set Iga
Thank you for your input, definitely very helpful! So you would recommend to use an immobilizing antibody, e.g. IgG Kit or Fab Kit instead of protein A/G? Or is direct immobilization better?
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