Imaging system to use instead of the X-ray film with a Southern blot, which employed the DIG High Prime DNA Labeling and Detection Starter Kit II?

02 February 2017 0 5K Report

I will do Southern blot using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche). Somebody can recommend me an imager or imaging system to observe the results, I don't want to use the X-ray film. Thanks.

Badges
Science method

More Alfredo Diaz-Lara's questions See All

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Journal of Research in Humanities and Social Science. Is a real magazine?

Dear colleagues: I have received many emails from a magazine called ournal of Research in Humanities and Social Science. I have gone to the website of this journal, and I read "some" indexes but,...

13 December 2020 6,208 6 View

Can you centrifuge and re-suspend a cDNA sample?

I am woking with very low concentrations of RNA in an attempt to run a qPCR analysis. Given this, I am going to attempt to synthesize cDNA without diluting my sample, and in two different,...

12 December 2020 3,247 3 View

How does climate change affect birds (shorebirds)?

I m recently investigating the impact of climate change on shorebird population trends, I would like to know what is the impact of fluctuations in variables such as sea surface temperature, global...

10 November 2020 9,293 15 View

How to create rarefaction curves in PAST?

I would like to make individual-based rarefaction curves in PAST software but struggle with creating the right input data table. I have 5 different sites, 7 sampling efforts on each (due to the...

25 October 2020 7,618 4 View

How do i obtain the APA references for the PDF articles ?

i need to reference my work for my honours degree in applied psychology and i cannot find the citations for each article i download. x

09 June 2020 7,437 3 View

Which is the best pipeline to do haplotype analysis in R?

I am studying the genetic diversity present in a collection of accessions for a few specific genes. I have VCF files as input data, and I am trying to use the R package 'pegas'. It seems really...

31 March 2020 588 3 View

Why can't I get target-specific sequences of Bryozoa in PCRs?

Hello, I am a PhD student interested in population genetics of marine invertebrates. Currently I'm working with a bryozoan: Reteporella. I have been trying to amplify COI (at least) and, although...

13 November 2019 7,387 4 View

New research into animal emotions?

Hi, I am trying to do research into new ways scientists are researching and understanding emotions in animals, (mammals, fish, birds, insects). Would anyone know of any interesting papers on the...

22 September 2019 9,686 12 View

Can anyone tell me how to access an instrument to measure social acceptance?

I have tried to access the following tools but have not been successful. Any suggestions or help is appreciated. 1. The Ohio Social Acceptance Scale 2. Adult Attachment Interview 3....

13 May 2019 4,572 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View