I will do Southern blot using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche). Somebody can recommend me an imager or imaging system to observe the results, I don't want to use the X-ray film. Thanks.
Hello all, I want to immortalize human adult fibroblasts with a lentivirus plasmid containing hTERT under the promoter EF1A and puromycin resistance under the promotor mPGK. I performed a...
14 May 2024 6,842 3 View
finance management
02 March 2024 5,278 4 View
Recently we’ve noticed these patterns on our separating gel 15% and I’m wondering if anyone can provide insight into it. Is it normal? And if it isn’t what could we possibly be doing wrong? We’ve...
28 January 2024 3,029 5 View
In comparison between simple distillation and vacuum distillation, in which one will the water boil more vigorously (large number of bubbles burst during boiling)?
20 January 2024 9,948 3 View
I am carrying an investigation with this title, therefore I need citations to support my research. I would like to review other investigations similar to mine, so that I can see what other...
17 December 2023 9,366 0 View
Dear colleagues, I hope this message finds you well. In our laboratory, we routinely conduct primary neuronal cultures derived from embryonic cortex, hippocampus, and spinal cord. Generally, our...
10 December 2023 1,883 1 View
When I tried to prepare a solution of enzalutamide of 1000 uM in cell culture medium from a solution of 1 M of enzalutamide in DMSO the drug precipitated. I've tried changing the pH and the...
08 December 2023 3,947 1 View
I have a question about the relationship between the cross-sectional area of an electrical transformer and the maximum power it can deliver. Empirically, the area is calculated by the square...
09 October 2023 1,259 4 View
Does the power consumption of heating mantle varies when I use it to heat boiling flask once filled with 1000 ml of water and once with 1400 ml water?! As the temperature controller knob remains...
06 October 2023 1,774 1 View
For organic liquid dye preparation to be used in cosmetic formulations
03 October 2023 6,047 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View