How to extract dye from dried red dragon fruit powder?

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All my transfected cell die after puromycin selection, wht to do?

Hello all, I want to immortalize human adult fibroblasts with a lentivirus plasmid containing hTERT under the promoter EF1A and puromycin resistance under the promotor mPGK. I performed a...

14 May 2024 6,842 3 View

Introduction to finance management?

finance management

02 March 2024 5,278 4 View

SDS-Gel separating gel polymerisation issues?

Recently we’ve noticed these patterns on our separating gel 15% and I’m wondering if anyone can provide insight into it. Is it normal? And if it isn’t what could we possibly be doing wrong? We’ve...

28 January 2024 3,029 5 View

In comparison between simple distillation and vacuum distillation?

In comparison between simple distillation and vacuum distillation, in which one will the water boil more vigorously (large number of bubbles burst during boiling)?

20 January 2024 9,948 3 View

Can somebody send me any investigation regarding Motivation to learn and teach English?

I am carrying an investigation with this title, therefore I need citations to support my research. I would like to review other investigations similar to mine, so that I can see what other...

17 December 2023 9,366 0 View

Any Advice about Unusual Structures in Primary Neuronal Cultures?

Dear colleagues, I hope this message finds you well. In our laboratory, we routinely conduct primary neuronal cultures derived from embryonic cortex, hippocampus, and spinal cord. Generally, our...

10 December 2023 1,883 1 View

How can I solve this problem?

When I tried to prepare a solution of enzalutamide of 1000 uM in cell culture medium from a solution of 1 M of enzalutamide in DMSO the drug precipitated. I've tried changing the pH and the...

08 December 2023 3,947 1 View

How is it determined and what is the basis for calculating the cross-sectional area that an electrical transformer should have?

I have a question about the relationship between the cross-sectional area of an electrical transformer and the maximum power it can deliver. Empirically, the area is calculated by the square...

09 October 2023 1,259 4 View

Does the Power Consumption of heating mantle varies?

Does the power consumption of heating mantle varies when I use it to heat boiling flask once filled with 1000 ml of water and once with 1400 ml water?! As the temperature controller knob remains...

06 October 2023 1,774 1 View

2 peaks on GC-MS have close retention time could be the same compound?

I analyzed spearmint essential oil sample, the chromatogram showed 2 peaks very close to each other, one of them at RT 13.74 detected as carvone, and the other one at RT 13.88 detected with name...

22 September 2023 1,729 2 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View