I'm working with Caco-2 cells for the first time. The TEER values are unchanged from day 3 to 21. Are the cells ready after only 3 days?

More Scott Sherrill's questions See All

Would anyone care to recommend an inexpensive waterproof serviceable (e.g., batteries can be changed) temperature logger?

I've been using Hobo pendants for years but they are up from less than $50 a few years ago to over $100, and I'm in need of an alternative.

08 July 2024 7,164 1 View

I submitted a Project Pitch to the NSF and I got approved and invited to submit a full proposal, how do I find researchers to contract?

I submitted a Project Pitch to the NSF and I got approved and invited to submit a full proposal, how do I find researchers to contract? It is for a novel heat engine so I will need someone with...

17 June 2024 6,487 1 View

Multinomial regression with spatial weigths in R?

Hi all, I am looking for a package in R that will allow me to run a multinomial logistic regression using spatial weights. I have run spatial autoregressive binary dependent variable models (SARB)...

22 May 2024 7,100 0 View

Tetramer binding not specific for HLA allele???

Hi All, Our group is doing some flow cytometry assays using an HLA-A*0201 tetramer presenting an HBV peptide (FLLTRILTI) and we're getting some strange results... We ran tetramer flow on a bunch...

16 May 2024 8,123 1 View

How do clinical researchers measure transference in psychotherapy patients/clients?

I have a hypothesis that the ceiling effects that appear on measures of the working alliance may be related to positive transference by patients/clients. But when I look into the research...

28 December 2023 4,740 7 View

Detecting free cysteine on proteins?

Does anyone know of a method to detect the number of free cysteines on proteins? I would like to assess the binding of quinones to cysteines on an enzyme.

17 November 2023 7,634 2 View

Have you observed cell division post-transfection?

I've been imaging mammalian cells (RPE-1) post-transfection and seen the transfected cells (fluorescent) become static and do not divide, whereas untransfected (non-fluorescent) cells in the same...

27 September 2023 9,524 1 View

What is the significance of harmonics in FFT of ECG ?

Attached image shows plot of Fast fourier transform of ECG that had applied a lowpass filter to (15 Hz, zero-phase shift FIR with 24 dB/octave roll-off). The frequency bands increase in increments...

21 August 2023 5,232 3 View

Should a material with a lower Tg have a lower Stiffness and modulus than one with higher Tg?

Tg vs stiffness and elastic modulus

20 July 2023 9,382 1 View

When is participatory action research not action research?

Would you consider a national study exploring the sources work related stress among a headteachers, and asking them what the solutions are, participatory / action research? Due to extent of the...

13 July 2023 6,877 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why do paired electrons atoms produce no magnetic field lines?

If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...

03 August 2024 3,927 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

Constanze Nossol

Hello,

it's difficult to say. How high are your values? ...You can check with immonuflourescence staining for tight junction proteins ZO-1, occludin, claudin 3 or 4...

Best regards,

Constanze

Lael Werner

How many cells did you plate?

Hui Ling Ma

I got the same problem recently, I always measured around 200 ohm from day 1 to day 15. I performed immunofluorescence, I can see the cell stained by DAPI and ZO-1 expression, however, there are some small holes.

Now, I suspect that the cells are mycoplasma contaminated. I am going to check it soon...