8 Questions 5 Answers 0 Followers
Questions related from Lael Werner
I am culturing CACO2 cells for experiments. I split them when they reach 80% confluence. what happens if they reach 100% confluence, and I keep splitting them? Confluence of CACO2 epithelial cells...
03 January 2024 9,220 1 View
Does someone have experience with expression of Tight junction molecules in FACS? I am looking for ZO-1 and occludin expression on CACO2 epithelial cells. Any suggestions or recommendations?
22 August 2023 5,341 0 View
Are there methods for Quantification of C. Albicans in serum or stool, without culturing them? Some fluorimetric or ELISA based assay? If it can differentiate between Yeast and Hypahe, even better
12 June 2023 8,819 2 View
I need to measure TEER in CACO2 cell, before and after 6 hours with some stimulation. How do I know if the cells are ready and confluent? In some articles, baseline TEER is 1000 OHM or higher. In...
29 March 2023 8,913 0 View
I am looking for a semi-qualitative method to determine microbial oxygen usage in human stool samples. In Wikipedia, Thioglycolate broth is used for the differentiation of obligate aerobes,...
22 January 2023 1,010 1 View
I am looking for Microbial Protease Inhibitor. HOWEVER. It has to be specific to bacteria. (it is in human stool samples which have both Microbial and Human Proteases. All Inhibitors I found are...
04 January 2023 8,882 1 View
How is it to differentiate Fecal Proteases if they are from human or bacterial source? There are no specific protease inhibitors for either?
14 April 2022 8,225 3 View
I am collecting samples from human Intestinal punch biopsies, taken at endoscopies. I am interested in placing biopsies in Organ Culture dishes, with luminal side (where epithelial cells reside)...
18 May 2021 1,172 0 View
