I'm looking for articles about water exported from natural peatlands (runoff), including quantification and chemical characterisation of these waters, can anyone help?
I need to know the possibilities of equipment for evaluating greenhouse gas emissions in peatlands of different states of conservation. Can anyone help me? Or alternatively, recognized...
06 February 2024 5,472 3 View
According with the Arrhenius equation the activation energy can be estimated determining the rate constant. Why often we see Arrhenius plots with the ln (reaction rate) as a funtion ln(1/T)?
09 November 2023 5,928 4 View
Good morning, I am trying to study the antioxidant activity of ZnO NPs. I have already performed some methods, such as DPPH and ABTS assay, but without success. When I search in the literature...
08 August 2023 3,876 1 View
As a 1st time user of BEAS-2B cells (human bronchial epithelial cells), which I'm starting to culture, I've noticed that they've been growing really slowly .... They were acquired by ATCC no...
06 December 2022 4,259 0 View
Good morning, I am trying to make the synthesis of ZnO NP with chitosan, but in the end of the process, the sample become like a stone/rock, I cannot destroy to powder, even with a pestle and...
17 November 2022 1,793 3 View
I did a transformation with a Golden Gate ESP3I reaction in DBH5 e coli. However, I got just one colony and it is very different from what I am used to. Instead of a circled format colony, I get a...
15 September 2022 6,570 2 View
I have a sample and in my study were going to compare 2 or 3 samples. If I am going to compare 2 groups, I need to know if one of the sample has a normal distribution and another doesn't, can I...
19 May 2022 2,304 7 View
I have used ethidium bromide solution for genotyping different mouse strains successfully, but the laboratory that I am working on currently does not allow it. Currently, I have been using a...
15 September 2021 6,860 3 View
I am wondering if these low levels of total RNA the samples are enough for RNA-seq. Does anyone already did it or has any suggestions to get a reliable data for bioinformatic analysis?
18 August 2021 3,891 7 View
Goodnight! I have questions regarding the definition of sample size. The aim of the study is to evaluate if the mother's diet will contribute to the offspring's obesity rates. The experiment in...
27 June 2021 3,485 4 View
I have incorporated the dispersion effect for the OPBE functional by specifying the S6, SR6 and S8 parameters for GD3 in Gaussian G09 package. For this purpose, I have defined the environment...
05 August 2024 1,494 2 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
23 July 2024 9,421 3 View
Phase quantification in steel contain BCC and FCC
20 July 2024 8,150 7 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is...
11 July 2024 2,257 1 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower...
11 July 2024 3,557 3 View
Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...
05 July 2024 4,197 1 View
Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...
01 July 2024 8,964 1 View