I'm doing a research about tissue culture and I think I don't have enough knowledge about it. What are the problems that I will face on my research and what are the solutions? What part of the plant will I use? Will I use MS medium or other mediums? What disinfectant will I use? I hope you can help me and thank you in advance.
Rajesh Dev Sarkar
If you are not that experienced, rather than going for callus induction and shoot generation, it is better to take axillary buds. Surface sterilize the buds with sodium hypochloride and culture in MS media. If you get so much of contamination, use cefotaxime.
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Seied Mehdi Miri
Dear Gerrard
You can use the micropropagation protocol of other species like Tectona grandis.
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