I stimulate the human PBMC with CpG for 6 hours and use Brefeldin A for inhibiting the IFN-alpha secretion. Then I just use the regular intracellular staining. I found that the Brefeldin A is better than Monensin at this case.

I've successfully stained for IFN-gamma in CD8+ T cells. Not sure if that would translate, but it may be worth a try.

I used the Foxp3 / Transcription Factor Staining Buffer Set from eBioscience to Fix/Perm activated splenocytes. Let me know if this is of interest to you.

Yeah I have had success with IFN-gamma too, but not with IFN-aplha

I stimulate the human PBMC with CpG for 6 hours and use Brefeldin A for inhibiting the IFN-alpha secretion. Then I just use the regular intracellular staining. I found that the Brefeldin A is better than Monensin at this case.

Dear Bishwas,

You can read this article.

Good luck.

CpG ODN Nanorings Induce IFNα from Plasmacytoid Dendritic Cells and Demonstrate Potent Vaccine Adjuvant Activity.

http://www.ncbi.nlm.nih.gov/pubmed/24807558