Could you please advise me that any suitable techniques for this experiment which will be done shortly?

I am planning to perform the functional study of gene whuch is supplied maternally, so morpholino-mediated knockdown assay won't work. Also about the CRISPR mediated knockout study need to wait...

27 December 2020 731 1 View

How do we apply a bending stiffness on to a wire model in abaqus?

I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...

01 July 2020 6,869 4 View

How to rectify the"DIsplacement Increment for contact is too big" warning in abaqus?

I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...

03 June 2019 4,811 5 View

How to generate adsorption isotherms ?

We are doing a project on adsorption of hexavalent chromium using agricultural wastes. I would want to generate adsorption isotherms for our data. Would I be able to know the procedure for the...

24 May 2019 1,655 13 View

Which is the good and required structure that need to be designed to solve current problems in a society?

I am a final year UG student, this semester we need to do design project. But we have learnt only member design in our curriculum, so I am looking for a structure by designing that we can get a...

15 June 2018 1,986 7 View

How can we calculate the series resistance and shunt resistance from J-V curve?

Which method is easier to calculate the series resistance and shunt resistance from the J-V curve measurements?Is there any softwares to get the values directly?

10 January 2018 1,139 1 View

BV2 cells - Unstable production of NO?

19 March 2017 224 3 View

What is a non-concrete material with very low tensile strength (~0.5-2 MPa)?

I'm looking to use a brittle material that is easy to mix and pour into small moulds with a very low tensile strength to investigate cracking behaviour of brittle hollow discs when subjected to an...

08 October 2015 6,471 8 View

How can I address the issue of a significant change in the Control group?

A study on effect of a particular yogic maneuver was carried out in our department. It involved two groups, control and intervention group( n = 50 in each). It so happened that, at the end of...

26 April 2015 4,409 3 View

What are the alternate materials for machine bases other than cast Iron ?

Material science are developing to great extent kindly share the information on alternative material for cast iron. I came to know that some of the machine bases are made of polymer concrete,...

04 February 2015 456 13 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Can I measure absorbance efficiency with wavelength in a spectrophotometer?

I measure the wavelength of material in a spectrophotometer and it peaks at 275 and its absorbance is 100. I mix it with an adsorbent and it adsorbs the first material, but when I measure the...

16 February 2021 7,515 5 View

Passivation of chromium cast Iron in high temperature?

14 February 2021 2,157 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

What dilution do I use for my GNP solution UV-Vis and DLS characterization?

I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...

26 January 2021 530 5 View

How to calculate DNA concentration of a sample with high concentration buffer?

I want to calculate the concentration of DNA in an unknown sample. But it's buffer has a high concentration and our nanodrop don't accept that as a blank of dsDNA. Thus I dilute the buffer with...

23 January 2021 9,440 2 View