How to improve results of system identification procedure?

Hello, I'm performing system identification of the lateral closed-loop dynamics of a quadrotor. My model receives a setpoint and should return position and acceleration. I've proposed a second...

07 February 2021 9,791 2 View

What is the maximum time duration allowed between photoresist coating and exposure?

I have coated the Silicon wafer with photoresist (AZ1518) but until the exposure (Laser Writer) I had to wait for more than a week (Laser Writer was not operative) - would the photoresist still be...

03 January 2021 9,640 4 View

What would happen if all nitrogen atoms in DNA were replaced by Carbon atoms ?

The DNA molecule is in the form of a double helix, The atoms which compose it are carbon, nitrogen, hydrogen, and phosphorus

02 January 2021 4,402 3 View

Which is more important for a journal? The impact factor or the quartile ranking?

I always found confusing both concepts! Which one do you think is more important? I give an example: The journal of "Theoretical and Applied Climatology" has an impact factor of 2.88, but it's a...

01 December 2020 8,608 19 View

Who can collaborate with us?

Salam (Hello)! We have prepared some new compounds with important potential to inhibit viruses. We are interested in inhibition of Hepatetis-B/C, Nipah, Nil and coronavirus. Who can collaborate...

22 November 2020 3,850 14 View

How to run an MGARCH-BEKK MODEL in eviews and interpret the results?

19 November 2020 4,350 3 View

Sir can you send me your research paper " Earthquake risk management assessment and evaluation for turkey" in short time?

05 November 2020 10,019 3 View

What is the most effective way to transfect HAP1 cells?

I am starting a project with the HAP1 cell line and previously our lab has had difficulty to transfect them. -we have nucleofection equipment - but have only been getting ~10% transfection -i...

19 October 2020 5,340 2 View

What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View