Is "thinking" an act of science?

When we think, what do we do? Do we philosophize or exercise science? Neither, either or both? How is thinking not science and how is thinking an act of science? How is it philosophy and how not a...

06 July 2019 1,268 5 View

What is theoretical Medicine?

Why cant there be a journal dedicated to the field of medicine which does not yet have any experimental basis much like theoretical medicine?

06 July 2019 1,238 9 View

Where can we submit/publish general philosophy essays?

There are limited places/portals/magazines that publish unsolicited general philosophy essays. Could anyone guide me how can I get my essays published on regular basis? Thank you

06 July 2019 6,326 2 View

How is artificial intelligence same or different from your intelligence?

How is artificial intelligence same or different from what we conventionally call natural intelligence?

06 July 2019 3,336 6 View

What does it mean to exist?

What does it actually mean to exist? How is it different from non-existence? How can we be sure if something exists or does not exist? I have made a case that these questions are more fundamental...

04 May 2019 5,109 24 View

Is slight illness is a good thing?

Are there any uses of illness or it is just all evil?

07 August 2018 3,086 1 View

Does "Sense of Humor" play any role in health and disease?

Does "Sense of Humor" play any role in health and disease?

07 August 2018 4,519 5 View

How to publish a hypothesis without experimental data?

How to publish a hypothesis without experimental data?

05 June 2018 5,196 8 View

Why is Mesentery considered an Organ?

Though Mesentery may be considered as a single entity all along the gut tube, but why is it called an organ? I don't see any reason for that. Neither is there any international body to come up...

05 June 2018 5,437 37 View

Difference between Dye and Stain?

What is the difference between Dye and Stain in Biological Experiments?

05 June 2018 995 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View