Bone marrow derived macrophages can be generated by M-CSF or L929 medium. But I don not know if there are some difference between them. I have used L929 medium to induce BMM, and found these BMM expressed high levels of CD206 (a marker of M2 macrophages). So L929 can contribute to the M2 polarization in contrast to M-CSF?
Thanks.
Hi!
I struggle a lot on the same question because I was working with two different benches of BMDMs, one differentiated with L929 and the other with M-CSF.
I was testing those cells with Flow Cytometry and I found that the expression of surface markers was different based on the differentiation method but also based on the cultivation methods, especially of MHC-II.
Cells seem to be more happy with L929 and attach better to the dish, however there could be something inhibiting the expression of some markers so I prefer to use M-CSF alone and treated dished to improve the quality and the vialibity of the cells.