Can water plus 0.02% merthiolate ?
Serum is at least a crude mixture of several proteases. Keep it frozen and in aliquots. The protein in the serum will protect your antibody sufficiently.
If not, add some NaN3 (0.01% and 1 mmol/L PMSF).
A simple conception of critical thinking requirements seems to be easier than the actual practice of the skill. What can be done to shift the whole project such that there are manifest outcomes of...
23 February 2024 6,497 3 View
Dear colleagues ! how which diagnostic of differentiating Infected from Vaccinated Animals (DIVA) of the Newcastle disease? Million thanks with Kind regards, Elvis
19 December 2023 6,845 2 View
What could be responsible for the non-amplication of a reference gene in a multiplex assay. The the other two targets amplified but the third (my reference gene) didn't. It however showed...
13 September 2023 2,931 0 View
I am using Taqman Assay Multiplex supplies the expression of 3 genes in my samples. The protocol says for each reach add: Master mix = 5 ul Dye Assay mix (Target 1) = 0.5 ul Dye Assay mix...
11 September 2023 7,772 3 View
I would like to find out if I may get research work on the impact of covid 19 on transport and mobility?
29 March 2022 9,398 11 View
Hi, we would like to measure the reduction potential of an enzyme. Can this be done via potentiometric titration? Our lab does not have cyclic voltammetry.
23 November 2021 4,708 2 View
Does anyone have any experiences on using the Thermo Fisher Accucore and Acclaim C30 HPLC columns? Are there any major differences between these two LC columns? I am wanting to separate 2...
18 November 2021 5,227 0 View
Currently, I am looking for some key literature (articles, book chapters, thesis, etc.) on mushroom leather and also identifying a suitable strain to engineer mycelium cells as they grow to create...
10 November 2021 3,447 4 View
Proteins generally absorb UV light at 280 nm while peptide bonds absorb UV light at 214 nm. When quantifying proteins using the Lowry and Buiret methods, absorbance or optical density is measured...
16 December 2020 1,325 3 View
I'm trying to compare several different surfactant efficiencies for Swage Fato Oil and Grease emulsification into water. I don't think that methods for fat determination in food for example...
06 October 2020 5,116 1 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
14 July 2024 3,106 3 View