Can water plus 0.02% merthiolate ?
Serum is at least a crude mixture of several proteases. Keep it frozen and in aliquots. The protein in the serum will protect your antibody sufficiently.
If not, add some NaN3 (0.01% and 1 mmol/L PMSF).
Proteins generally absorb UV light at 280 nm while peptide bonds absorb UV light at 214 nm. When quantifying proteins using the Lowry and Buiret methods, absorbance or optical density is measured...
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I'm trying to compare several different surfactant efficiencies for Swage Fato Oil and Grease emulsification into water. I don't think that methods for fat determination in food for example...
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The three personality characteristics that are documented in almost all entrepreneurship documented information are: i. Need for Achievement ii. Internal Locus of Control iii. Risk-taking Why in...
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Are the researchers doing enough to assist to the betterment of our society?
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Good afternoon. I have a problem detecting proteins on my gel after SDS PAGE. After extracting the protein, i quantified using the Lowry's method and had concentrations ranging between 2 to...
29 August 2016 5,994 4 View
I have six constructs with the following G-C contents: 29.6%, 23.2%, 27.2%, 24.4%, 27% and 28%. Due to the low G-C content of genes of interest, the Tm of my primers (see attached file) are low;...
03 March 2015 9,011 9 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
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Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
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Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
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We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
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I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
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Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
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I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
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When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
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I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
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I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View