12 December 2018 10 6K Report

Dear all, I am currently working on antimicrobial mechanisms of Campylobacter and i want to ascertain whether tetO gene were located in the chromosome within the CJSA_0191 locus or located on pTet plasmid. Thus i used 2 primers, one used to generate an

amplicon size of approximately 2000bp and it was successfully identified and the other one designed based on the pTet sequence were employed for an amplicon size of about 3500bp and i tried many times to amplify this gene but failed to do so. please guide me.

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