Eman Bassiony

4 Questions 37 Answers 0 Followers

Questions related from Eman Bassiony

How to measure the level of expression of eGFP in cells ?

Hello, I am looking for a method to detect the strength of expressed eGFP signals into cells other than qPCR, I am using the UV microscope to recognize the fluorescence but want to determine the...

03 March 2019 2,318 5 View

Promoters Regions Identification for Subsequent Cloning?

Hi everyone I need to clone a promoter region to an expression vector for further heterologous gene expression. I am trying to identify the gene region first (the one containing the promoter I...

11 November 2017 4,299 4 View

Combining genes for eGFP in heterologous gene expression?

Hi everone, I noticed that in many gene expression experiments, people combine the gene of interest to eGFP, Does anybody know the advantage of this method ? Is it only for easy detection of the...

08 August 2017 2,167 3 View

What is the best way for storing plasmids?

Hello everybody, Is it Ok to store it at -20 or better to store it in glycerol stocks at -80 ? and how to recover it again from glycerol to be used in cloning? and how many days or weeks can I...

10 October 2016 3,056 6 View