How to solve carry-over and interference problem when detecting tulathromycin by LCMS?

I am working on the determination of tulathromycin in rat faeces using QE-focus (Thermo Fisher)(Waters BEH C18, 2.1x100mm, 1.7um)(0.1%FA+H2O, 0.1%FA+ACN) . I encountered two tricky problems....

02 January 2021 2,713 5 View

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21 October 2020 7,860 3 View

Can anyone share image dataset of food such as cheese, fruits, one object per photo?

I need a dataset of food items. Prefer one object per photo. Any hints are helpful.

05 October 2020 536 5 View

Electropolymeriztaion to synthesis polymer film. But it is too thin, what to do?

Hey guys, these days I am using electropolymerization to form polymer film. I mimiced the procedure of the paper, and got very thin membrane (~hundreds of nanometer), while the paper says they...

24 August 2020 3,379 7 View

Is there a gentle method to reduce cuprous oxide to copper while maintaining the original morphology?

Hello, Recently, I have synthesized some cuprous oxide nanomaterials and want to reduce them to metal while maintaining the morphology. Is there a way to do this?

04 August 2020 4,850 3 View

What is the best way to register crop point clouds and spectral information in a plot of the field ?

Hi everyone, The goal: i want to register crop point clouds and spectral information in a plot of the field. The data detail: 1.The crop point clouds will be obtained by terrestrial Lidar (or UAV...

05 July 2020 9,128 5 View

The collagen gel evaporate with 3D organoid model (air Liquid interface method), why?

Hi, guys, I have a queation, please help me. I used air liquid interface method to do 3D culture. This is the first time to do it. the collagen gel prepared according to literature, 8 vol of...

13 May 2020 7,288 2 View

Can someone recommend me how to plot such pie chart for analyzing antibody germline usage and somatic hypermutations?

I would like to analyze the germline usage and somatic hypermutations of a large number of antibody sequences, can somebody recommend a software or R package to generate such pie chart plot...

28 March 2020 9,574 2 View

How often to get more loci/SNPs from reference based compare to de novo in RADSeq?

Hi, I have this simple but wired question that how many of you ever compared the results of the same data set using both reference-based and de novo approach, and get more loci/SNPs using the...

16 February 2020 9,641 4 View

Can pramipexole be used instead of apomorphine for the verification of 6-hydroxydopamine induced Parkinson's disease mice model?

since apomorphine is not availble, and pramipexole and apomorphine are both dopamine receptor agonists, so I'm wondering if I can use other dopamine receptor agonists to verify the rotation test...

08 January 2020 152 3 View

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02 March 2021 3,060 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View