Xiao Yh

3 Questions 8 Answers 0 Followers

Questions related from Xiao Yh

What will happen if I extract plasmids from some competent strains that alreadly contains an innate plasmid?

Greetings, I have recently been considering the creation of a new competent E.coli strain for further research. I'm interested in incorporating a plasmid into this strain(~10Kb in size |...

11 August 2023 4,866 5 View

Why this enzymatic reaction do not have increased reaction rate with substrate concentration increases?

Recently I am stuggling to improve the kinetics of an artifical enzyme. I expressed this enzyme in E.coli and then test it's kinetics properties. I noticed that if I pick single conies for...

23 May 2023 2,269 6 View

If it is proper to substitude NaCl with KCl in IP buffer for plant?

Thanks for click in. Recently I'm performing plant cell lysis and IP with IP buffer, and I noticed that KCl at 100mM in IP buffer can provide a better result in all means. And I wonder whether it...

01 April 2019 5,160 1 View