Most of the cells differentiate and proliferate using Growth Factors. Is it possible that same chemotaxis be used to strengthen the tissue by triggering greater collagen production in the cells due to the presence of collagen in the ECM ?
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...
07 July 2024 8,671 6 View
I'm working with HEI-OC1 cells of the inner ear. Recently I'm seeing these round particles in the flask (picture attached), but cells' morphology and growth seems normal. However, there are...
26 June 2024 8,717 7 View
Hello, I aim to compare the Length of Stay (LOS) in hours b/n two independent groups, with sample sizes of 300 and 310. The LOS data does not follow a normal distribution and shows right-skewed...
25 June 2024 1,842 7 View
Hi everyone, I was wondering how to get the correlation between one dependent variable and independent variable, Pearson correlation is not an option as there is a follow up visit for each...
24 June 2024 8,104 6 View
Hi everyone, we know that the analysis of pilot studies should be based on descriptive statistics and ideally not involve any inferential statistics (i.e., P values). Based on this can I use Roc...
24 June 2024 4,402 7 View
The Analyse for Pilot study should be based on the descriptive stat and ideally not involved inferential stat. what about exploratory study? can I do inferential stat like cox regression or...
17 June 2024 5,470 3 View
Hi everyone, I've created a p-chart to monitor a binary variable on a quarterly basis. But I noticed a p-chart requires at least 20 data points. So, I've shifted to monthly reporting. with this...
06 June 2024 4,849 0 View
Hi everyone, what assumption will be used if the assumption of one-way Anova are unmet? I think Kruskal Wallis-test, am I right? What about two-way Anova?
03 June 2024 2,220 3 View
I have two outcome variables: 1- binary 2- repeated measure There is only one independent variable which is time varying covariate Iam looking for the article to write the Stat for: 1-...
01 June 2024 9,890 3 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
What can you look for to know that the circuit model being used is overfitting the data. How do you know when overfitting is occurring?
31 July 2024 7,425 0 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
Hi, I will run an experiment where reaction time will be the dependent variable, tested in 2 conditions (with the same participants). I want to control for a confounding variable (individual...
20 July 2024 1,943 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View