Most of the cells differentiate and proliferate using Growth Factors. Is it possible that same chemotaxis be used to strengthen the tissue by triggering greater collagen production in the cells due to the presence of collagen in the ECM ?
I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...
15 November 2020 8,556 3 View
in vitro mice CD8 cell activation. These cells are extracted from B6 mice spleen by magnetic separation.
01 November 2020 3,276 2 View
I have 2 drugs (PTC-209 and GSK343) that are soluble in DMSO (I already made the stock solution, dissolved to a clear solution) and I have to inject them IP to mice for a 2 week treatment course....
17 September 2020 9,107 3 View
In 1993, Lent et al. introduced one of the most promising and emerging computational paradigm known as Quantum-dot Cellular Automaton (QCA) computing. Now, it is almost 30 years have been passed,...
06 May 2020 4,955 7 View
During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?
04 May 2020 4,700 11 View
I am looking to communicate with a personnel who deals with beginner-level review articles, and want to learn how to write good reviews and the rules and regulations to follow.
06 March 2020 6,068 3 View
We know that EDTA is used by different reasons (chelating agent, support for antimicrobial effectiveness e.g) in pharmaceuticals. Is there any specific reason to prefer EDTA in injectables...
01 December 2019 5,166 3 View
Hi everyone, I am working on simulation of stirred tank reactor operating at batch mode. So, there is no fluid inlet-outlet in the system. Can I calculate the local mean age of fluid by using...
18 November 2019 1,142 3 View
I wish to know what aspects of these technical documents are included and excluded while making a word count. Specifically, say, a figure caption, references, tables, table footnotes, page...
24 June 2019 9,815 3 View
I have ordered a pair of primers, forward primer is working well, I have checked it by gel, but revers primer has really weak band in gel, and PCR doesn't work. any suggestion?
18 November 2018 5,958 3 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...
10 February 2021 5,863 2 View
I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...
07 February 2021 5,487 3 View
I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...
05 February 2021 2,530 3 View
Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...
05 February 2021 7,276 1 View