If i use anhydrous chemical instead of hydrous to prepare synthetic medium for thermophilic bacteria fermentation, Can bacteria grow or not?

30 January 2023 1 6K Report

I need to prepare a synthetic medium. In the literature, they used di-potassium hydrogen orthophosphate trihydrate. In my lab, di-potassium hydrogen orthophosphate ( anhydrous ) is available. can I use it? TIA

Richard Symes

This should not affect bacterial growth so long as you account for the different in molecular mass.

i.e. 1 gram of the trihydrate is the same as 0.763 gram of the anhydrous.

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