I perform real time PCR for a cancer patient to check the NIS expression levels where I use Gapdh as an reference gene. How can I calculate ddCT in this context as I do not have the tissue from the respective tumor sample. Or will relative expression be enough to give an explanation while writing a paper?
Andrew W. Tai
You can't obtain a ddCt from a single sample, if I am understanding your question correctly. The question you would use ddCt for is whether NIS expression changes (up or down) in one tissue/cell sample versus another, relative to a standard gene whose expression should not change (in your case GAPDH).
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