If i have an extract in aquades, and i want to determine antioxidant activity using DPPH in etanol, should i remove the solvent (aquades)?

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Abhishek Sharma Popular answer

Use DPPH assay; Take ethanol also as control and subtract ethanol if it scavange.

Shivani Pandya

Dear Kharishma,

You can use the extract directly with the ratio of 1:1 of your extract in aquades and DPPH in ethanol. For such purpose take baseline of 1:1 aquades and ethanol to determine scavenging activity using UV-Vis spectrophotometry .

Abhishek Sharma

Veerabhadrappa Kv

Dear Karishma,

You can use the extract by concentrating reduce the volume of aquades in extract and dissolve in dmso at the same time DPPH disolve in the ethanol, do scavenging activity by UV-Vis spectrophotometry .