Remove 200 ml of chloroplasts, being sure to mix the suspension well before removing the sample. Dilute this suspension 1:100 with isolation buffer to make a dilute chloroplast suspension. I want a total of 15mL
initial and final states, where "f" is final and "i" is initial.
Solve for Vf (final volume):
Say initial concentration: Ci = X
Say final concentration: Cf = (1 / 100) * X = X / 100
Vf = (Ci / Cf) * Vi
= [X / (X / 100)] * 200 µL
= 100 * 200µL
= 20,000 uL
= 20 mL
Take 200µL of chloroplast solution at some "X" concentration and add 19.8 mL of isolation buffer to make a (1 / 100) dilution. You have 20 mL worth of this dilute solution. That is enough.