If a gene of a particular bacteria is not sequenced and reported in NCBI, how can I prepare primer?

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Rajesh Kumar Meher

To prepare primer you should have gene sequence data other wise you can try by taking RAPD primer.

All the best

Timir Tripathi

U can find conserved sequences and then make degenerate primers. Then u have to do random amplification of cDNA ends to amplify and reach the starting atg and stop codons. Sequence and your gene will be there.

Rabbul Ibne A. Ahad

Thank you so much sir for your valuable suggestions. Can you suggest me how to find conserved sequence?