What is/ are the significance of pseudo first order and pseudo second order reaction in metal removal?

How the kinetic models interpret the metal removal process?

04 May 2019 2,765 5 View

If a gene of a particular bacteria is not sequenced and reported in NCBI, how can I prepare primer?

Gene sequence of cadA1 and cadA2 of cyanobacteria is not reported in NCBI. I want to prepare primer these two gene. How to proceed to make the primers in a effective way !!! Please suggest.

08 September 2018 5,983 3 View

What is the difference between cite score and impact factor of a journal?

08 September 2017 7,739 10 View

Is there any modulator which modulate Cu efflux in cyanobacteria?

Cyanobacterial cells have efflux system to maintain ionic balance of the cells.Is there any modulators reported in literature which modulate Cu efflux in cyanobacteria or any other bacteria ?????

01 January 1970 5,007 3 View

How Cu/ Cd precipitates at pH 8.0? Is there any chemical to prevent metal (Cu and Cd) precipitation?

How to prevent Cu/ Cd precipitation in medium at pH 8.0 or more than that pH. How many researchers perform different experiments at pH 8.0 or 9.0?? Please suggest some approach to prevent metal...

01 January 1970 6,590 7 View

Is there any fluorescence based method to detect and quantify metal transporters?

01 January 1970 1,026 2 View

What is the significance of Temkin parameters in adsorption of metal?

01 January 1970 6,596 11 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

How best to model transportation during usage phase of LCA for a mobile water tank in SimaPro (kg*km)?

I would like to model the usage phase of a mobile water tank. The tank is transported on a tractor or lorry so I need to model the transportation too. My problem is that when I select a...

01 February 2021 6,759 3 View