If a gene of a particular bacteria is not sequenced and reported in NCBI, how can I prepare primer?

Gene sequence of cadA1 and cadA2 of cyanobacteria is not reported in NCBI. I want to prepare primer these two gene. How to proceed to make the primers in a effective way !!! Please suggest.

08 September 2018 5,983 3 View

What is the difference between cite score and impact factor of a journal?

08 September 2017 7,739 10 View

Is there any modulator which modulate Cu efflux in cyanobacteria?

Cyanobacterial cells have efflux system to maintain ionic balance of the cells.Is there any modulators reported in literature which modulate Cu efflux in cyanobacteria or any other bacteria ?????

01 January 1970 5,007 3 View

Can you send me detailed list of metal efflux transporters bacteria/ cyanobacteria

Can any one send the me the list of metal efflux transporters specifically for Cd and Cu transporters.

01 January 1970 8,530 1 View

How Cu/ Cd precipitates at pH 8.0? Is there any chemical to prevent metal (Cu and Cd) precipitation?

How to prevent Cu/ Cd precipitation in medium at pH 8.0 or more than that pH. How many researchers perform different experiments at pH 8.0 or 9.0?? Please suggest some approach to prevent metal...

01 January 1970 6,590 7 View

Is there any fluorescence based method to detect and quantify metal transporters?

01 January 1970 1,026 2 View

What is the significance of Temkin parameters in adsorption of metal?

01 January 1970 6,596 11 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View