Hi Grandhi,

Do you have a local puff tube(spritzer or equivalent) which can put in small volumes of drugs locally and can be washed off very effectively.

You can start your spontaneous/evoked recordings, achieve a stable condition and then apply the drug locally near a responsive electrode and compare the activity with a controlled electrode. Finally washi it of for understanding the reversibility of the drug.

This can be done multiple times depending on the number of responsive electrodes and reversibility of the drug.

Regards,

Debanjan

Thanks for the suggestions.

We do not have local puff tube as you discussed.

We are using micro electrode arrays (64 electrodes) using MEA2100 system from Multi Channel Systems, Germany.

We usually perfuse the slices with ACSF maintained at 32 0C from a bath continuously supplied with carbogen gas.

Best Regards,

Grandhi V Ramalingayya

I guess you only have the option of bath perfusing a drug and washing it of after sometime.

However, you would have some circuit level artifacts ie due to the activity on certain neurons which might be connecting to and hence activating some other neurons on the dish.

But to start with its not a bad idea.

Regards,

Debanjan

Sorry. These very interesting questions are out of my competence. Michael.

See Ghoochani et al., 2016 Oncotarget.

Best, Nic