Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

I put a sodium chloride which is less than solubility chart but it doesn't melt. can you tell me what the problem is?

24 September 2020 224 3 View

Does anyone has a cif file for Pt-Ni alloy metal?

Hello. I am trying to fit the EXAFS data of Pt-Ni (around 1:1) alloy nano particles on oxide support. - How can i fit the Pt-Ni metallic peak which is a bit different from Ni metal and Pt metal...

21 July 2020 8,044 3 View

How to check the existence of pinholes in ZnO thin films?

I fabricated a devices whose structure is "bottom electrode(ITO thin film sputterted)" - ZnO thin film - "top electrode(Ag thin film)". The thickness of ZnO thin film is about 200nm. The ZnO thin...

10 March 2019 9,591 3 View

Is it possible to use part unit of HPLC/MSD?

Our lab has used the LC/MS(LCQ-Deca XP,Thermo, it is still working condition and not walking with PC). LC(Shiseido) is working as stand alone now. Recently, it disconnect to MSD. MSD is not...

19 February 2018 8,547 3 View

Is it ok that the Al thin film is transparent?

Hello. I'm curious about the transparency of Al thin film with sputtering method. I made Aluminium(Al) thin film with sputtering method which has ~ 10nm thickness on a transparent substrate....

18 December 2017 9,651 11 View

Why is nanowire better than thin film in piezoelectric nanogenerator?

19 August 2017 6,980 1 View

Should two fluorescent dyes contact for happening FRET?

18 April 2017 1,644 2 View

Is it possible to use Prep HPLC as casual HPLC?

25 May 2016 3,213 7 View

HPLC column selection question?

25 October 2015 2,973 8 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View