I would like to know the volume of lining fluid in mice
Dear Cesar,
Recently I found a review saying that we still don't have a method for quantification of lung lining fluid:
"There are, however, no alternatives to in vivo determinations of lung surface area and lung lining fluid."
Find the citation below,
Article Measurements of Deposition, Lung Surface Area and Lung Fluid...
Hope it helps,
Marcos
You may find this paper helpful:
https://journals.physiology.org/doi/full/10.1152/jappl.2000.89.2.672?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
Thank you so much!!!!
Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...
17 February 2021 2,896 3 View
I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!
11 February 2021 7,273 3 View
I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...
11 February 2021 1,328 3 View
I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....
24 January 2021 5,988 2 View
I need a free of charge and short questionnaire that measures mood states. The questionnaire will be used as in a neuropsychological evaluation study. It is well known that mood states can...
16 January 2021 8,284 4 View
Usually industrial policy is interpreted as changing economic structure from in favor of more productive/advanced economic activities. But would like to read something that discusses the...
31 December 2020 3,199 3 View
I want to do a descriptive analysis using the World Values Survey dataset which has an N=1200. However, even thought I have searched a lot, I haven't found the methodology or a tool to calculate...
17 November 2020 1,505 4 View
Hi, i'm looking for bibliographic references that explains the relationship between plant hormones and plant allometry (relation between the size of an organism and aspects of its physiology and...
21 September 2020 9,653 6 View
In an experiment I found that the fresh weight of the root was less than that of the stem, however when I found the dry weight, the weight of the stem was less than that of the root. Why are these...
20 September 2020 2,269 16 View
I have been trying to use NEB's TtAgo (https://www.neb.com/products/m0665-ttago) to linearize supercoiled plasmids, following NEB's recommendations, but I haven't had any success yet (19/19 failed...
09 September 2020 2,112 1 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...
02 March 2021 1,999 5 View
during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...
01 March 2021 7,476 4 View
I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...
01 March 2021 6,985 3 View
I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...
01 March 2021 8,668 3 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
How can one improve mesh skewness, orthogonality, aspect ratio?
28 February 2021 7,730 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View