If you work in the laboratory setting, or in the diagnostic field, it is very likely that you use Streptavidin or NeutrAvidin. I would like to hear your opinion and the experiences on the difference between NeurAvidin vs. Streptavidin
Hi! I have a question. I was looking into the research question: does mohs micrographic surgery give less recurrences and metastasis than standard excision in the treatment of cuteanous squamous...
17 June 2024 7,769 5 View
Hello, I am working on an sEEG project and I am trying to figure out how to convert coordinates of my depth electrodes that I have in native/subject space into MNI space so I can use multiple...
05 June 2022 3,133 0 View
Does anyone know where I can find publicly available travel surveys from low and middle income countries and cities? Travel surveys such as travel diaries (with trip purpose, mode, time, possibly...
11 October 2021 922 6 View
Hi so I need help with finding how to get grants as a student. So I graduated this year and am planning on doing a Masters By Research in Botswana, primarily focusing on Leopards and estimating...
06 October 2021 1,443 3 View
I run a multilevel model (unconflated multilevel model) with multiple dependent variables that are highly correlated (>.90). A reviewer mentioned that in this way the unique relationships with...
31 October 2020 499 2 View
Very clinically relevant to reduce stress and related obesity
26 January 2020 8,873 4 View
I want to use Unconflated Multilevel Modeling (UMM) to test a 1-1-1 model in which the effects of the mediating and predictive variables can also be on level 2. Because of a small sample size (164...
24 December 2019 8,912 3 View
In my research I hypothised that team learning mediates between transformational leadership and innovative work behavior. To analyse the relations I use HLM 7. I found a significant positive...
25 January 2018 10,128 4 View
I'm searching for recent studies in the field of international/intercultural communication related to the opinion leadership concept or similar papers (educational policy, gatekeeper etc.)
14 June 2015 5,749 5 View
I might be overly sensitive here but I am scared that my precious tissue samples are now useless for RNA extraction. The tissues are not stored in RNAlater or a similar reagent. The temperature...
25 March 2014 950 9 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
What is the best way to obtain high-performance aptamers after performing sequence amplification by asymmetric PCR other than biotin and streptavidin?
17 April 2024 6,973 2 View
Does avidin binds to streptavidin?
14 April 2024 5,692 1 View
I'm having a problem with intense background noise in immunofluorescence for my biotinylated protein of interest conjugated with streptavidin (attached with a fluorophore) in mouse liver tissue...
11 April 2024 6,395 0 View
I have a question for the professionals. The essence of the problem: I fix the oligos on a plastic substrate, they play role of primers in solid-phase PCR. I carry out a one-step PCR with...
28 March 2024 9,110 2 View
Hi, I want to study RNA interacting with my test compound I am planning to do a RNA- pulldown experiment using streptavidin magnetic beads and biotin conjugated compound. I will detect...
22 February 2024 8,684 1 View
I have labeled my replicating bacterial DNA with 5-Ethynyl-2'-deoxyuridine (EdU) and want to click it with biotin-PEG-3-azide followed by enrichment with magnetic streptavidin beads. So far, I...
06 February 2024 5,381 0 View
Hi, I want to perform chromatography on viruses which are larger than the protein targets people typically use chromatography columns on. Because of this I need to use 2% agarose beads which...
30 October 2023 2,771 3 View
Hello, we have been struggling with the lots of background in our wb membranes probed with an anti-Streptavidin-HRP from Thermofisher (Pierce 21134). Samples contained biotinilated proteins. Every...
16 October 2023 3,778 2 View
My peptide has biotin attached to it, and I am trying to bind that to streptavidin agarose beads. After 1-4 hrs of mixing, when washing with 5X DPBS, almost all the biotin-peptide comes off. Are...
02 October 2023 1,559 0 View