If you work in the laboratory setting, or in the diagnostic field, it is very likely that you use Streptavidin or NeutrAvidin. I would like to hear your opinion and the experiences on the difference between NeurAvidin vs. Streptavidin
I want to use Unconflated Multilevel Modeling (UMM) to test a 1-1-1 model in which the effects of the mediating and predictive variables can also be on level 2. Because of a small sample size (164...
24 December 2019 8,603 3 View
In my research I hypothised that team learning mediates between transformational leadership and innovative work behavior. To analyse the relations I use HLM 7. I found a significant positive...
25 January 2018 9,913 4 View
I might be overly sensitive here but I am scared that my precious tissue samples are now useless for RNA extraction. The tissues are not stored in RNAlater or a similar reagent. The temperature...
25 March 2014 869 9 View
We always see an increase of the two tautomeric forms of Tacrolimus in a 80% ethanol solution. Is there a way to get Tacrolimus more stable?
09 January 2014 4,346 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
We are fabricating acrylamide beads as probe substrate. How can we make streptavidin bind to the surface of the acrylamide? Thanks!
09 December 2020 8,141 7 View
I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...
20 September 2020 730 2 View
I will be adding streptavidin onto the surface of a graphene coated glass slide; thereafter, I will be analyzing with a MALDI-TOF. I was wondering the best way to go about this? I was thinking of...
07 September 2020 5,902 4 View
I am working with a florescent compound that is biotinylated. When strepdavidin is present in the system, the binding of the protein with the compound quenches the florescence. I need to perform...
16 August 2020 6,937 8 View
Hello! I have been trying to understand this for more than a year now, and I am at a loss. Can anyone help, please?? So, I follow this protocol :Statistical Correlation Between SERS Intensity...
31 May 2020 658 10 View
I want to synthesize positively charged gold nanoparticles and then surface decorate with streptavidin. The ideal size would be 20nm or less. Please help.
18 March 2020 7,955 2 View
The wells are polystyrene coated with streptavidin via a biotin BSA linking layer using the SAC well coating method. What is SAC coating method?
06 March 2020 3,016 3 View
I have 1Kd biotin tagged peptide. The alone peptide is running at the expected size but when I am allowing it to bind to streptavidin beads it's running around 10Kd. What could be the reason?
02 March 2020 1,681 3 View
Hi, everyone! I'm trying to select HEK293FS cells expressing SBP-LNGFR protein. The protein presence on the cell membrane is confirmed with immunofluorescence. But the MACS procedure with...
03 February 2020 5,791 4 View