We are working with SH-SY5Y for many years but did not face problems like this. Cells morphology have changed and the freeze that we are keeping at -80 or even in liquid nitrogen are not attaching to the plates. Can anybody suggest something?
Did anything change in your freezing protocol? It happened to me that failure to inactivate the trypsin properly before freezing killed one of my batches. Maybe you over-trypsinized?
Hi Russa, it is absolutely normal for SH-SY5Y cells to form clumps or even float around in the medium sometimes. You can collect the medium, centrifuge it for a few minutes, discard the supernatant and add fresh medium then add back to the adherent cells.