Xiang-Bin Wang

2 Questions 7 Answers 0 Followers

Questions related from Xiang-Bin Wang

I want to use CAS9 systems to knock in expression cassette into CHO cells to make stable cell lnes. how do I choose the hot spot for knocking in?

Cas9 is a good genome editing tool. I want to make stable cell lines using CAS9 knocking in tech. I don't know how to choose hot spot for inserting. Does anybody know about it?

26 September 2014 1,595 2 View

Does methylation affect the CMV promoter in large scale expression of mAb?

We have established several cell lines for expressing mAb with GS/CHO systems. The expression level decreased obviously in > 50L bio-reactors. Some papers say that it's because of the effect of...

17 September 2014 2,978 5 View