I want to take CHO cells in culture. For that I need for each passaging of cells in an sterile Erlenmeyer flask. After washing, the Erlenmeyer flasks are autoclaved with a lid, dried at 37°C and treated for a time with a UV lamp. Nevertheless, I have a problem of bacterial contamination in some bottles.
Can anyone give tips?
https://tools.thermofisher.com/content/sfs/manuals/FreeStyle_CHO_S_Cells_QR.pdf