30 November 2015 3 10K Report

I want to take CHO cells in culture. For that I need for each passaging of cells in an sterile Erlenmeyer flask. After washing, the Erlenmeyer flasks are autoclaved with a lid, dried at 37°C and treated for a time with a UV lamp. Nevertheless, I have a problem of bacterial contamination in some bottles. 

Can anyone give tips?

https://tools.thermofisher.com/content/sfs/manuals/FreeStyle_CHO_S_Cells_QR.pdf

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