I want to show the binding between Pol-l-lysine and glucose oxidase or any other similar type enzyme using two fluorescent dyes. Dye for tagging?

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H3K9Me2/3 western blots in S.pombe?

I have recently started working with heterochromatin . I am trying to quantify the H3K9Me levels but i am failing to do so. Sometimes i see faint duplex band . I do TCA Lysis and then follow up...

22 May 2024 2,071 2 View

Whether electric field and magnetic field can be oscillated in the opposite co-ordinate direction in the case of the unpolarized EMR?

In the Cartesian Corredinate commonly the direction of the electric field is towards the Y axis and magnetic field is towards the Z axis and the propagation is towards the X axis of EMR. Here, I...

25 September 2023 5,340 2 View

Is it possible to calculate PLQY for Solid Powder samples using PL ?

(Metal halide Perovskites)

14 September 2023 9,957 0 View

Is it possible to calculate PLQY for Solid Powder samples using PL ?

(Metal halide Perovskites)

14 September 2023 7,623 0 View

Why am I seeing a broad DPV peak from 0.2V to -0.2V when I use mod-GCE with 0.1M acetate buffer?

I have been trying to run a DPV experiment using modified Glassy carbon electrode as the working electrode, Pt as the counter and Ag/AgCl as the reference. Before modifying the electrode, I have...

01 August 2023 8,059 0 View

What are the methods can be performed in SPSS for validation of Logistic regression (when the model fits the data)?

I have been performed the multinomial logistic regression model in SPSS. The goodness of fit shows that the model fits the data. Based on my literature study, there are several methods can be...

30 July 2023 9,281 5 View

Whether Quadrantal Bearing and Reduced Bearing is same or different?

I was discussing with the students in my class on whole circle bearing and quadrantal bearing. They asked me that whether quadrantal bearing and reduced bearing is same or different. I had...

28 June 2023 5,875 4 View

What is the best strategy to get rid of endophytes when using woody stem as explants from plants maintained in arboretum?

I am trying to induce callus from woody plants grown in an arboretum. We have tried sterilizing explants using bleach but we are unable to get rid of funal contaminations. The explants are woody...

22 April 2023 1,704 2 View

How could a linear regression model be validated using SPSS or STATA? How to result out the consistency or accuracy of the same?

Kindly help.

24 March 2023 7,328 3 View

How to have different threshold for different images?

I am doing edge detection of microcell images directly taken form microscope. One image has 25 microwells containing the cells. I am cropping the 25 automatically and trying to do edge detection....

06 December 2022 3,933 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View