I want to present HCMV peptides to monocytes and then see the T cell activation. I want to know monocyte and Lymphocyte concentration?

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Plant tissue culture: ammonium sulfate instead of ammonium nitrate? what concentration?

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Protocol for induction of shooting from callus chickpea?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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Julie Joseph

This paper has the general protocol (co-culture): Article Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells...

This one has used a sequential approach (which is what you may want): https://www.frontiersin.org/articles/10.3389/fimmu.2013.00389/full#h3

I am sure there are a lot more protocols out there that deal with antigen presentation.

Gnaneshwar Jadav

Thank you very much