The dendritic cells will not proliferate cause they are already differentiated . The cells are from fibrotic mice liver. I plan on using the cells for immunofluorescence. I will be using 4-well chamber slides from Ibidi.
Hi Fanta,
It’s better to culture the cells at a lower density for immuno-fluorescence unless the microscopic images would be too crowded with the cells. You haven’t mentioned the type of your culture dish. I will give you optimized cell densities for few culture dishes.
1) 4-chamber slide – 25 000 - 30 000 cells/chamber
2) 24 well plate – 30 000 – 35 000 cells/well
3) 6 well plate – 140 000 – 150 000 cells/well
Plating at the densities above will make the plate 75-80% confluent by 48h. In other words, these densities are calculated for 24h incubation after seeding and 24h incubation after the sample treatment. If you wish to incubate the cells more than 24h with the samples, yo may need to reduce the above densities accordingly.
Good Luck,
Susara
Hi Fanta,
It’s better to culture the cells at a lower density for immuno-fluorescence unless the microscopic images would be too crowded with the cells. You haven’t mentioned the type of your culture dish. I will give you optimized cell densities for few culture dishes.
1) 4-chamber slide – 25 000 - 30 000 cells/chamber
2) 24 well plate – 30 000 – 35 000 cells/well
3) 6 well plate – 140 000 – 150 000 cells/well
Plating at the densities above will make the plate 75-80% confluent by 48h. In other words, these densities are calculated for 24h incubation after seeding and 24h incubation after the sample treatment. If you wish to incubate the cells more than 24h with the samples, yo may need to reduce the above densities accordingly.
Good Luck,
Susara