I want to perform Antioxidant assay for my cyanobacterial sample ,can any body suggest complete protocol for the assay like POD,SOD,CAT,APX?

More Niharika Sahu's questions See All

Can anyone suggest papers in the hybrid auto-mata area that models cyber-physical systems in power systems?

Most of the hybrid automata papers target formal verification of the systems. I am looking for papers that are solving optimal response problems that propose optimal control in power systems under...

07 February 2021 5,233 1 View

What is the recommended age limit for primary posterior capsulotomy in pediatric cataract surgery?

In our centre, we are doing PPC even for older children upto 10-12 years of age and there seem to be no harm, rather beneficial and good outcomes. Please share your experiences.

27 December 2020 4,268 6 View

Increase coercivity with device size (multi-domain) ?

I'm working with magnetic material particularly CoFeB. I notice a weird pattern. I see that my coercivity increases with device size. The device is multi domain and contrary to what I'd expect....

09 December 2020 1,577 1 View

Software for L x T mating design analysis and heterosis estimation?

Kindly suggest the name of free softwares available for LxT analysis and heterosis estimation in crop plants.

22 October 2020 1,744 10 View

I have performed XRD on Fusion zone of 316L stainless steel as well as incoloy 800. Why only one or two peaks are showing?

I am facing problem as only one or two peaking are coming while performing XRD on Fusion Zone of 316L SS and alloy 800. I want to know where i am committing mistake.I have attached the XRD image.

07 October 2020 6,205 3 View

Please suggest the name of important and free softwares for attractive NJ tree, dendrogram and PCA diagram?

I would like to do genetic diversity analysis. Kindly suggest the name of important and free softwares for attractive NJ tree, dendrogram and PCA diagram?

30 September 2020 4,684 7 View

Band decomposed charge density using quantum espresso ?

Dear sir/mam Greetings for the day ! (1). I am using quantum espresso(QE) to calculate band decomposed charge density. To my knowledge, the kpoint at which we want to calculate the charge...

21 August 2020 1,095 2 View

How to find out the Compund Average Annual Publications Growth Rate (CAGR) in Bibliometrics ?

I need an explanation with example and calculation.

30 July 2020 8,765 1 View

What is the best graph based database for storing complex graphs from diverse domain?

This question is related to making inferences from a complex graphical model. Graph databases are used to store and query the relationship between nodes in a graph. We need an open-source graph DB...

18 June 2020 5,903 3 View

Is rationality normative? Can rationality be naturalized? Is rationality depended on normative conditions, or is it independent of normativity?

Rationality is itself an elusive term. There are debates on the definition and criteria of rationality. The primary assumption of the naturalized and non-naturalized rationality on normative...

05 May 2020 1,095 78 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Mohamed Sabri Bensaad

Dear Niharika,

I hope the Antioxydant protocols found in these 2 articles (attached) will help you in your research.

Best wishes

Niharika Sahu

thank you sir for your suggestion.

Praveen Kumar

Please find the below link. You can get more ideas about it...

https://rdcu.be/co1RA

Ahad Madani

please see my paper in MAYDICA (2016)