I want to knock out a gene in the U87 cell line. Can you suggest a gene that causes aggression (invasion and metastasis)?

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Malcolm Nobre

Hello Nisa

You mentioned it right. Hsc70 could be knocked out in U87 cell line. You can refer to the research paper below.

Article Overexpression of Hsc70 promotes proliferation, migration, a...

The other gene which you could consider is cripto-1. You can refer to the research article below for more details.

Article Cripto‑1 overexpression in U87 glioblastoma cells activates ...

Regards.

Nisa Devrim

Thank you.

Aras Emre Canda

Dear Devrim, I don't have a specific experience with knockout genes. I recommend Professor Yasemin Baspinar and Associate Professor Gizem Kocal for discussion. Best.