I want to have a agar disk diffusion assay for antimicrobial compounds. E coli is the target organism . How much E coli do I need to plate out?

More Janitha Senevirathna's questions See All

Can someone help me to find Q1 & Q2 Journals please?

My research area is “influence of technological advancements in fashion retail physical interior stores” need to find literature relating to this from Q1 and Q2 journals. Can someone help me, please?

11 March 2024 1,826 0 View

Which instrument is suitable for measure intrinsic viscosity (IV) of PET ?

27 September 2023 9,304 0 View

Constrained strain, Stress recovery ?

Hello, Is it possible to run constrain strain, stress recovery through Discovery HR-2 (TA Instruments). Please advice me. Thank You, Janitha.

03 September 2021 1,440 0 View

After chromatographic separation, does it need to evaporate mobile phase in order carryout bioassay and NMR?

I going to check the bioactivities of plant extracts

20 June 2021 9,685 9 View

Can you suggest a method to isolate compounds from plant extract and to determine their structure ?

I have a plant extract and I want to isolate metabolites present in it. Which chromatographic method and spectroscopy will be ideal for this

14 June 2021 8,273 18 View

Hi, Can you suggest a suitable spectroscopic technique to elucidate structure of metabolite molecule?

I want to determine the structure of extracellular metabolite of plant extract

14 June 2021 1,761 18 View

What is the significant difference beween two DSC curves?

Dear All, The bisphenol A based epoxy was cured under two curing cycles (curing cycle I and curing cycle II). After that, conducted a DSC experiment, and the results attached herewith. Why both...

02 May 2021 9,280 4 View

Dynamic mechanical analyser (DMA) test standards ?

Hello, I found that many researchers used, different settings (Heating rate, frequency, thickness… etc.) in DMA testings. To obtain glass transition temperature(Tg), what is the standard we...

27 September 2019 9,846 5 View

What is the alternate option for multipile linear regression analysis in a cross sectional study?

i want to assess the associations between the independent and outcome variable by using multiple linear regression model. Is there any alternate to this?

17 July 2017 5,193 2 View

Does anyone have any questionnaire pertaining to competency for lecturers?

26 August 2016 9,379 7 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View